LOCUS       Exported                8153 bp ds-DNA     circular SYN 27-MAR-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    portmage3
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8153)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, March 27, 2017 from SnapGene Viewer 3.1.2
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8153
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    complement(149..1996)
                     /label=mutL E32K
                     /note="mutL E32K"
     misc_feature    complement(2014..2694)
                     /label=exo
                     /note="exo"
     misc_feature    complement(2691..3476)
                     /label=bet
                     /note="bet"
     misc_feature    complement(3482..3778)
                     /label=gam
                     /note="gam"
     promoter        complement(3848..3892)
                     /label=pL promoter
                     /note="pL promoter"
     misc_feature    complement(4526..5320)
                     /label=KanR
                     /note="KanR"
     misc_feature    complement(5588..6301)
                     /label=cI repressor
                     /note="cI repressor"
     misc_feature    6435..8153
                     /label=pBBRI origin
                     /note="pBBRI origin"
ORIGIN
        1 cagctgtctc ttatacacat ctccgctgtg ctttcagtgg atttcggata acagaaaggc
       61 cgggaaatac ccagcctcgc tttgtaacgg agtagacgaa agtgattgcg cctacccgga
      121 tattatcgtg aggatgctca tcgccatttc actcatcttt cagggctttt atcgccggat
      181 gtaaatcaac agattgtaac agaccacccg gcggcgtttt cacaagttgc ggacataacc
      241 gttccacgtc cgccagcagg gttatggcct gtgccattga ccactgcgca tgttcgctca
      301 tcagatttcg tgcaatccac tgcgcaatat tgccaggttc gaatacggac tgcttcgcca
      361 ggtagcctat cagttcagga atcaagattt gtaaattttg ttggcgtaag ggtaaaggca
      421 ctgccctgat ggtcacatgc tgtgcatctg actggaaatc aatacccaat tccgccaggg
      481 cagactgcgc tttttctaat gccgattttt cttcggcaga aacttttagc cgcaacggaa
      541 tcagcagcgg ctgggcgcaa acgggcgctt cacccggcgt caattgtgcc tgacgcagcc
      601 aacgttctgc cactggcaag gataaaagtg aaatgttgcc gtcgcgctcc agcaacgcac
      661 agtcggaatg gacgatagtc agtacccgac caaaactctg actgttcgcc gcaagtgcag
      721 gttcctgcgg ttccggcgct tttaattttt gcatcggcgc gggcgtttgc aaaagctggc
      781 gatacacttc accttgctgt ttctggtagc ctggctgcgc attcggccag ggggcagccg
      841 gacgactgcc tgatgctggc gcaggagtgt agcgcggagc taccggctca cgagctgccg
      901 gttctgcaaa gtgattgcgc cccgccgcca cgcggttttc cggaatggaa cgcggtgcag
      961 gttggggttc atcgtccagc ggtagcggcg tttccagttg ctgttgtagc acgctcagca
     1021 cgccctgata gataaaatca tgcaccagac gcgactgatg gaaacgcact tcgtgtttgg
     1081 cggggtgcac gttgacgtcc acctgatgtg ggtcgatctc cagatacaac acaaatgccg
     1141 gttgctgatc ggcccccagt ttgtcttcgc aggcctggcg gatcgcgtga ttgatcaggc
     1201 gatcgcgcat catgcgaccg ttcacgtagc aatactgaat ttctgccagt gcgggcgtgg
     1261 tgtgatttgg atcggccacc cagccgcgta gcgtgagatc gccgtgttgc cattcaatcg
     1321 ccagcgcttg ttcaagaaaa gcggtgccgc aaatcgcgcc taagcgccgt tctttttgcc
     1381 cgccttccgg cactgcgcgg tactgacgca caattttacc gttatgcgac aggttgatcg
     1441 tgacgtcgaa acgcgccagc gcaatgcggc ggatgatctc atcaatgtgg ttaaattcgg
     1501 ttttctcggt gcgcaggaat ttgcgccgcg ccggggtgtt gtagaacaga tccagcacct
     1561 ccagcgtcgt ccccacagga tgcgccgccg gttttaccgt cacgttcata tcgcgccctt
     1621 cggcataggc ctgccaggct tcctgctgtt ctgcggtgcg tgaagtgagc gtcaggcggg
     1681 aaaccgaact gatactcgcc agcgcctcac cgcgaaagcc caggctgata atggcttcga
     1741 gatcgtccag agaggcgatt ttactggtgg catgacgagc cagcgccagc gccagctcat
     1801 cttttttgat accgcagccg ttatcacgaa tgcggataag tttcgcccca ccgcgttcga
     1861 tatcaatatc gatacgcgtc gcacctgcat cgaggctgtt tttcactagt tctttgacta
     1921 ccgacgcagg tcgctcgacc acctcacctg cggcaatctg gttcgccagt tgtggcggta
     1981 agacctgaat tggcattttg taatcctcct ctctcatcgc cattgctccc caaatacaaa
     2041 accaatttca gccagtgcct cgtccatttt ttcgatgaac tccggcacga tctcgtcaaa
     2101 actcgccatg tacttttcat cccgctcaat cacgacataa tgcaggcctt cacgcttcat
     2161 acgcgggtca tagttggcaa agtaccaggc attttttcgc gtcacccaca tgctgtactg
     2221 cacctgggcc atgtaagctg actttatggc ctcgaaacca ccgagccgga acttcatgaa
     2281 atcccgggag gtaaacgggc atttcagttc aaggccgttg ccgtcactgc ataaaccatc
     2341 gggagagcag gcggtacgca tactttcgtc gcgatagatg atcggggatt cagtaacatt
     2401 cacgccggaa gtaaattcaa acagggttct ggcgtcgttc tcgtactgtt ttccccaggc
     2461 cagtgcttta gcgttaactt ccggagccac accggtgcaa acctcagcaa gcagggtgtg
     2521 gaagtaggac attttcatgt caggccactt ctttccggag cggggttttg ctatcacgtt
     2581 gtgaacttct gaagcggtga tgacgccgag ccgtaatttg tgccacgcat cttccccctg
     2641 ttcgacagct ctcacatcga tcccggtacg ctgcaggata atgtccggtg tcatgctgcc
     2701 accttctgct ctgcggcttt ctgtttcagg aatccaagag cttttactgc ttcggcctgt
     2761 gtcagttctg acgatgcacg aatgtcgcgg cgaaatatct gggaacagag cggcaataag
     2821 tcgtcatccc atgttttatc cagggcgatc agcagagtgt taatctcctg catggtttca
     2881 tcgttaaccg gagtgatgtc gcgttccggc tgacgttctg cagtgtatgc agtattttcg
     2941 acaatgcgct cggcttcatc cttgtcatag ataccagcaa atccgaaggc cagacgggca
     3001 cactgaatca tggctttatg acgtaacatc cgtttgggat gcgactgcca cggccccgtg
     3061 atttctctgc cttcgcgagt tttgaatggt tcgcggcggc attcatccat ccattcggta
     3121 acgcagatcg gatgattacg gtccttgcgg taaatccggc atgtacagga ttcattgtcc
     3181 tgctcaaagt ccatgccatc aaactgctgg ttttcattga tgatgcggga ccagccatca
     3241 acgcccacca ccggaacgat gccattctgc ttatcaggaa aggcgtaaat ttctttcgtc
     3301 cacggattaa ggccgtactg gttggcaacg atcagtaatg cgatgaactg cgcatcgctg
     3361 gcatcacctt taaatgccgt ctggcgaaga gtggtgatca gttcctgtgg gtcgacagaa
     3421 tccatgccga cacgttcagc cagcttccca gccagcgttg cgagtgcagt actcattcgt
     3481 tttatacctc tgaatcaata tcaacctggt ggtgagcaat ggtttcaacc atgtaccgga
     3541 tgtgttctgc catgcgctcc tgaaactcaa catcgtcatc aaacgcacgg gtaatggatt
     3601 ttttgctggc cccgtggcgt tgcaaatgat cgatgcatag cgattcaaac aggtgctggg
     3661 gcaggccttt ttccatgtcg tctgccagtt ctgcctcttt ctcttcacgg gcgagctgct
     3721 ggtagtgacg cgcccagctc tgagcctcaa gacgatcctg aatgtaataa gcgttcatgg
     3781 ctgaactcct gaaatagctg tgaaaatatc gcccgcgaaa tgccgggctg attagtaatc
     3841 cggaatcgca cttacggcca atgcttcgtt tcgtatcaca caccccaaag ccttctgctt
     3901 tgaatgctgc ccttcttcag ggcttaattt ttaagagcgt caccttcatg gtggtcagtg
     3961 cgtcctgctg atgtgctcag tatcaccgcc agtggtattt atgtcaacac cgccagagat
     4021 aatttatcac cgcagatggt tatctgtatg ttttttatat gaatttattt tttgcagggg
     4081 ggcattgttt ggtaggtgag agatctgaat tgctatgttt agtgagttgt atctatttat
     4141 ttttcaataa atacaattgg ttatgtgttt tgggggcgat cgtgaggcaa agaaaacccg
     4201 gcgctgaggc cgggttaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     4261 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     4321 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     4381 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     4441 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttaa tgaagttttg
     4501 acggtatcga accccagagt cccgctcaga agaactcgtc aagaaggcga tagaaggcga
     4561 tgcgctgcga atcgggagcg gcgataccgt aaagcacgag gaagcggtca gcccattcgc
     4621 cgccaagctc ttcagcaata tcacgggtag ccaacgctat gtcctgatag cggtccgcca
     4681 cacccagccg gccacagtcg atgaatccag aaaagcggcc attttccacc atgatattcg
     4741 gcaagcaggc atcgccatgg gtcacgacga gatcctcgcc gtcgggcatc cgcgccttga
     4801 gcctggcgaa cagttcggct ggcgcgagcc cctgatgctc ttcgtccaga tcatcctgat
     4861 cgacaagacc ggcttccatc cgagtacgtg ctcgctcgat gcgatgtttc gcttggtggt
     4921 cgaatgggca ggtagccgga tcaagcgtat gcagccgccg cattgcatca gccatgatgg
     4981 atactttctc ggcaggagca aggtgagatg acaggagatc ctgccccggc acttcgccca
     5041 atagcagcca gtcccttccc gcttcagtga caacgtcgag cacagctgcg caaggaacgc
     5101 ccgtcgtggc cagccacgat agccgcgctg cctcgtcttg gagttcattc agggcaccgg
     5161 acaggtcggt cttgacaaaa agaaccgggc gcccctgcgc tgacagccgg aacacggcgg
     5221 catcagagca gccgattgtc tgttgtgccc agtcatagcc gaatagcctc tccacccaag
     5281 cggccggaga acctgcgtgc aatccatctt gttcaatcat gcgaaacgat cctcatcctg
     5341 tctcttgatc cactagatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     5401 atattctctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     5461 gatacatatt tgaatgaatt caaccattta ctatgttatg ttctgagggg agtgaaaatt
     5521 cccctaattc gatgaagatt cttgctcaat tgttatcagc tatgcgccga ccagaacacc
     5581 ttgccgatca gccaaacgtc tcttcaggcc actgactagc gataactttc cccacaacgg
     5641 aacaactctc attgcatggg atcattgggt actgtgggtt tagtggttgt aaaaacacct
     5701 gaccgctatc cctgatcagt ttcttgaagg taaactcatc acccccaagt ctggctatgc
     5761 agaaatcacc tggctcaaca gcctgctcag ggtcaacgag aattaacatt ccgtcaggaa
     5821 agcttggctt ggagcctgtt ggtgcggtca tggaattacc ttcaacctca agccagaatg
     5881 cagaatcact ggcttttttg gttgtgctta cccatctctc cgcatcacct ttggtaaagg
     5941 ttctaagctc aggtgagaac atccctgcct gaacatgaga aaaaacaggg tactcatact
     6001 cacttctaag tgacggctgc atactaaccg cttcatacat ctcgtagatt tctctggcga
     6061 ttgaagggct aaattcttca acgctaactt tgagaatttt tgtaagcaat gcggcgttat
     6121 aagcatttaa tgcattgatg ccattaaata aagcaccaac gcctgactgc cccatcccca
     6181 tcttgtctgc gacagattcc tgggataagc caagttcatt tttctttttt tcataaattg
     6241 ctttaaggcg acgtgcgtcc tcaagctgct cttgtgttaa tggtttcttt tttgtgctca
     6301 tacgttaaat ctatcaccgc aagggataaa tatctaacac cgtgcgtgtt gactatttta
     6361 cctctggcgg tgataatggt tgcatgtact aaggaggttg tatggaacaa cgagatgtgt
     6421 ataagagaca gctggcctgc ccctcccttt tggtgtccaa ccggctcgac gggggcagcg
     6481 caaggcggtg cctccggcgg gccactcaat gcttgagtat actcactaga ctttgcttcg
     6541 caaagtcgtg accgcctacg gcggctgcgg cgccctacgg gcttgctctc cgggcttcgc
     6601 cctgcgcggt cgctgcgctc ccttgccagc ccgtggatat gtggacgatg gccgcgagcg
     6661 gccaccggct ggctcgcttc gctcggcccg tggacaaccc tgctggacaa gctgatggac
     6721 aggctgcgcc tgcccacgag cttgaccaca gggattgccc accggctacc cagccttcga
     6781 ccacataccc accggctcca actgcgcggc ctgcggcctt gccccatcaa tttttttaat
     6841 tttctctggg gaaaagcctc cggcctgcgg cctgcgcgct tcgcttgccg gttggacacc
     6901 aagtggaagg cgggtcaagg ctcgcgcagc gaccgcgcag cggcttggcc ttgacgcgcc
     6961 tggaacgacc caagcctatg cgagtggggg cagtcgaagg gcgaagcccg cccgcctgcc
     7021 ccccgagcct cacggcggcg agtgcggggg ttccaagggg gcagcgccac cttgggcaag
     7081 gccgaaggcc gcgcagtcga tcaacaagcc ccggaggggc cactttttgc cggaggggga
     7141 gccgcgccga aggcgtgggg gaaccccgca ggggtgccct tctttgggca ccaaagaact
     7201 agatataggg cgaaatgcga aagacttaaa aatcaacaac ttaaaaaagg ggggtacgca
     7261 acagctcatt gcggcacccc ccgcaatagc tcattgcgta ggttaaagaa aatctgtaat
     7321 tgactgccac ttttacgcaa cgcataattg ttgtcgcgct gccgaaaagt tgcagctgat
     7381 tgcgcatggt gccgcaaccg tgcggcaccc ctaccgcatg gagataagca tggccacgca
     7441 gtccagagaa atcggcattc aagccaagaa caagcccggt cactgggtgc aaacggaacg
     7501 caaagcgcat gaggcgtggg ccgggcttat tgcgaggaaa cccacggcgg caatgctgct
     7561 gcatcacctc gtggcgcaga tgggccacca gaacgccgtg gtggtcagcc agaagacact
     7621 ttccaagctc atcggacgtt ctttgcggac ggtccaatac gcagtcaagg acttggtggc
     7681 cgagcgctgg atctccgtcg tgaagctcaa cggccccggc accgtgtcgg cctacgtggt
     7741 caatgaccgc gtggcgtggg gccagccccg cgaccagttg cgcctgtcgg tgttcagtgc
     7801 cgccgtggtg gttgatcacg acgaccagga cgaatcgctg ttggggcatg gcgacctgcg
     7861 ccgcatcccg accctgtatc cgggcgagca gcaactaccg accggccccg gcgaggagcc
     7921 gcccagccag cccggcattc cgggcatgga accagacctg ccagccttga ccgaaacgga
     7981 ggaatgggaa cggcgcgggc agcagcgcct gccgatgccc gatgagccgt gttttctgga
     8041 cgatggcgag ccgttggagc cgccgacacg ggtcacgctg ccgcgccggt agcacttggg
     8101 ttgcgcagca acccgtaagt gcgctgttcc agactatcgg ctgtaccgcc tcg
//
