LOCUS       Exported                8101 bp ds-DNA     circular SYN 27-MAR-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    portmage4
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8101)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, March 27, 2017 from SnapGene Viewer 3.1.2
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8101
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    complement(149..1996)
                     /label=mutL E32K
                     /note="mutL E32K"
     misc_feature    complement(2014..2694)
                     /label=exo
                     /note="exo"
     misc_feature    complement(2691..3476)
                     /label=bet
                     /note="bet"
     misc_feature    complement(3482..3778)
                     /label=gam
                     /note="gam"
     promoter        complement(3848..3892)
                     /label=pL promoter
                     /note="pL promoter"
     misc_feature    complement(4516..5175)
                     /label=cat
                     /note="cat"
     misc_feature    complement(5536..6249)
                     /label=cI repressor
                     /note="cI repressor"
     misc_feature    6383..8101
                     /label=pBBRI origin
                     /note="pBBRI origin"
ORIGIN
        1 cagctgtctc ttatacacat ctccgctgtg ctttcagtgg atttcggata acagaaaggc
       61 cgggaaatac ccagcctcgc tttgtaacgg agtagacgaa agtgattgcg cctacccgga
      121 tattatcgtg aggatgctca tcgccatttc actcatcttt cagggctttt atcgccggat
      181 gtaaatcaac agattgtaac agaccacccg gcggcgtttt cacaagttgc ggacataacc
      241 gttccacgtc cgccagcagg gttatggcct gtgccattga ccactgcgca tgttcgctca
      301 tcagatttcg tgcaatccac tgcgcaatat tgccaggttc gaatacggac tgcttcgcca
      361 ggtagcctat cagttcagga atcaagattt gtaaattttg ttggcgtaag ggtaaaggca
      421 ctgccctgat ggtcacatgc tgtgcatctg actggaaatc aatacccaat tccgccaggg
      481 cagactgcgc tttttctaat gccgattttt cttcggcaga aacttttagc cgcaacggaa
      541 tcagcagcgg ctgggcgcaa acgggcgctt cacccggcgt caattgtgcc tgacgcagcc
      601 aacgttctgc cactggcaag gataaaagtg aaatgttgcc gtcgcgctcc agcaacgcac
      661 agtcggaatg gacgatagtc agtacccgac caaaactctg actgttcgcc gcaagtgcag
      721 gttcctgcgg ttccggcgct tttaattttt gcatcggcgc gggcgtttgc aaaagctggc
      781 gatacacttc accttgctgt ttctggtagc ctggctgcgc attcggccag ggggcagccg
      841 gacgactgcc tgatgctggc gcaggagtgt agcgcggagc taccggctca cgagctgccg
      901 gttctgcaaa gtgattgcgc cccgccgcca cgcggttttc cggaatggaa cgcggtgcag
      961 gttggggttc atcgtccagc ggtagcggcg tttccagttg ctgttgtagc acgctcagca
     1021 cgccctgata gataaaatca tgcaccagac gcgactgatg gaaacgcact tcgtgtttgg
     1081 cggggtgcac gttgacgtcc acctgatgtg ggtcgatctc cagatacaac acaaatgccg
     1141 gttgctgatc ggcccccagt ttgtcttcgc aggcctggcg gatcgcgtga ttgatcaggc
     1201 gatcgcgcat catgcgaccg ttcacgtagc aatactgaat ttctgccagt gcgggcgtgg
     1261 tgtgatttgg atcggccacc cagccgcgta gcgtgagatc gccgtgttgc cattcaatcg
     1321 ccagcgcttg ttcaagaaaa gcggtgccgc aaatcgcgcc taagcgccgt tctttttgcc
     1381 cgccttccgg cactgcgcgg tactgacgca caattttacc gttatgcgac aggttgatcg
     1441 tgacgtcgaa acgcgccagc gcaatgcggc ggatgatctc atcaatgtgg ttaaattcgg
     1501 ttttctcggt gcgcaggaat ttgcgccgcg ccggggtgtt gtagaacaga tccagcacct
     1561 ccagcgtcgt ccccacagga tgcgccgccg gttttaccgt cacgttcata tcgcgccctt
     1621 cggcataggc ctgccaggct tcctgctgtt ctgcggtgcg tgaagtgagc gtcaggcggg
     1681 aaaccgaact gatactcgcc agcgcctcac cgcgaaagcc caggctgata atggcttcga
     1741 gatcgtccag agaggcgatt ttactggtgg catgacgagc cagcgccagc gccagctcat
     1801 cttttttgat accgcagccg ttatcacgaa tgcggataag tttcgcccca ccgcgttcga
     1861 tatcaatatc gatacgcgtc gcacctgcat cgaggctgtt tttcactagt tctttgacta
     1921 ccgacgcagg tcgctcgacc acctcacctg cggcaatctg gttcgccagt tgtggcggta
     1981 agacctgaat tggcattttg taatcctcct ctctcatcgc cattgctccc caaatacaaa
     2041 accaatttca gccagtgcct cgtccatttt ttcgatgaac tccggcacga tctcgtcaaa
     2101 actcgccatg tacttttcat cccgctcaat cacgacataa tgcaggcctt cacgcttcat
     2161 acgcgggtca tagttggcaa agtaccaggc attttttcgc gtcacccaca tgctgtactg
     2221 cacctgggcc atgtaagctg actttatggc ctcgaaacca ccgagccgga acttcatgaa
     2281 atcccgggag gtaaacgggc atttcagttc aaggccgttg ccgtcactgc ataaaccatc
     2341 gggagagcag gcggtacgca tactttcgtc gcgatagatg atcggggatt cagtaacatt
     2401 cacgccggaa gtaaattcaa acagggttct ggcgtcgttc tcgtactgtt ttccccaggc
     2461 cagtgcttta gcgttaactt ccggagccac accggtgcaa acctcagcaa gcagggtgtg
     2521 gaagtaggac attttcatgt caggccactt ctttccggag cggggttttg ctatcacgtt
     2581 gtgaacttct gaagcggtga tgacgccgag ccgtaatttg tgccacgcat cttccccctg
     2641 ttcgacagct ctcacatcga tcccggtacg ctgcaggata atgtccggtg tcatgctgcc
     2701 accttctgct ctgcggcttt ctgtttcagg aatccaagag cttttactgc ttcggcctgt
     2761 gtcagttctg acgatgcacg aatgtcgcgg cgaaatatct gggaacagag cggcaataag
     2821 tcgtcatccc atgttttatc cagggcgatc agcagagtgt taatctcctg catggtttca
     2881 tcgttaaccg gagtgatgtc gcgttccggc tgacgttctg cagtgtatgc agtattttcg
     2941 acaatgcgct cggcttcatc cttgtcatag ataccagcaa atccgaaggc cagacgggca
     3001 cactgaatca tggctttatg acgtaacatc cgtttgggat gcgactgcca cggccccgtg
     3061 atttctctgc cttcgcgagt tttgaatggt tcgcggcggc attcatccat ccattcggta
     3121 acgcagatcg gatgattacg gtccttgcgg taaatccggc atgtacagga ttcattgtcc
     3181 tgctcaaagt ccatgccatc aaactgctgg ttttcattga tgatgcggga ccagccatca
     3241 acgcccacca ccggaacgat gccattctgc ttatcaggaa aggcgtaaat ttctttcgtc
     3301 cacggattaa ggccgtactg gttggcaacg atcagtaatg cgatgaactg cgcatcgctg
     3361 gcatcacctt taaatgccgt ctggcgaaga gtggtgatca gttcctgtgg gtcgacagaa
     3421 tccatgccga cacgttcagc cagcttccca gccagcgttg cgagtgcagt actcattcgt
     3481 tttatacctc tgaatcaata tcaacctggt ggtgagcaat ggtttcaacc atgtaccgga
     3541 tgtgttctgc catgcgctcc tgaaactcaa catcgtcatc aaacgcacgg gtaatggatt
     3601 ttttgctggc cccgtggcgt tgcaaatgat cgatgcatag cgattcaaac aggtgctggg
     3661 gcaggccttt ttccatgtcg tctgccagtt ctgcctcttt ctcttcacgg gcgagctgct
     3721 ggtagtgacg cgcccagctc tgagcctcaa gacgatcctg aatgtaataa gcgttcatgg
     3781 ctgaactcct gaaatagctg tgaaaatatc gcccgcgaaa tgccgggctg attagtaatc
     3841 cggaatcgca cttacggcca atgcttcgtt tcgtatcaca caccccaaag ccttctgctt
     3901 tgaatgctgc ccttcttcag ggcttaattt ttaagagcgt caccttcatg gtggtcagtg
     3961 cgtcctgctg atgtgctcag tatcaccgcc agtggtattt atgtcaacac cgccagagat
     4021 aatttatcac cgcagatggt tatctgtatg ttttttatat gaatttattt tttgcagggg
     4081 ggcattgttt ggtaggtgag agatctgaat tgctatgttt agtgagttgt atctatttat
     4141 ttttcaataa atacaattgg ttatgtgttt tgggggcgat cgtgaggcaa agaaaacccg
     4201 gcgctgaggc cgggttaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     4261 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     4321 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     4381 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     4441 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttaa ggcaccaata
     4501 actgccttaa aaaaattacg ccccgccctg ccactcatcg cagtactgtt gtaattcatt
     4561 aagcattctg ccgacatgga agccatcaca gacggcatga tgaacctgaa tcgccagcgg
     4621 catcagcacc ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa
     4681 gttgtccata ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg gattggctga
     4741 gacgaaaaac atattctcaa taaacccttt agggaaatag gccaggtttt caccgtaaca
     4801 cgccacatct tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca
     4861 gagcgatgaa aacgtttcag tttgctcatg gaaaacggtg taacaagggt gaacactatc
     4921 ccatatcacc agctcaccgt ctttcattgc catacggaat ttcggatgag cattcatcag
     4981 gcgggcaaga atgtgaataa aggccggata aaacttgtgc ttatttttct ttacggtctt
     5041 taaaaaggcc gtaatatcca gctgaacggt ctggttatag gtacattgag caactgactg
     5101 aaatgcctca aaatgttctt tacgatgcca ttgggatata tcaacggtgg tatatccagt
     5161 gatttttttc tccattttag cttccttagc tcctgaaaat ctcgataact caaaaaatac
     5221 gcccggtagt gatcttattt cattatggtg aaagttggaa cctcttacgt gccgatcaac
     5281 gtctcatttt cgccaaaagt tggcccaggg cttcccggta tcaacaggga caccaggatt
     5341 tatttattct gcgctcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     5401 catgagcgga tacatatttg aatgaattca accatttact atgttatgtt ctgaggggag
     5461 tgaaaattcc cctaattcga tgaagattct tgctcaattg ttatcagcta tgcgccgacc
     5521 agaacacctt gccgatcagc caaacgtctc ttcaggccac tgactagcga taactttccc
     5581 cacaacggaa caactctcat tgcatgggat cattgggtac tgtgggttta gtggttgtaa
     5641 aaacacctga ccgctatccc tgatcagttt cttgaaggta aactcatcac ccccaagtct
     5701 ggctatgcag aaatcacctg gctcaacagc ctgctcaggg tcaacgagaa ttaacattcc
     5761 gtcaggaaag cttggcttgg agcctgttgg tgcggtcatg gaattacctt caacctcaag
     5821 ccagaatgca gaatcactgg cttttttggt tgtgcttacc catctctccg catcaccttt
     5881 ggtaaaggtt ctaagctcag gtgagaacat ccctgcctga acatgagaaa aaacagggta
     5941 ctcatactca cttctaagtg acggctgcat actaaccgct tcatacatct cgtagatttc
     6001 tctggcgatt gaagggctaa attcttcaac gctaactttg agaatttttg taagcaatgc
     6061 ggcgttataa gcatttaatg cattgatgcc attaaataaa gcaccaacgc ctgactgccc
     6121 catccccatc ttgtctgcga cagattcctg ggataagcca agttcatttt tctttttttc
     6181 ataaattgct ttaaggcgac gtgcgtcctc aagctgctct tgtgttaatg gtttcttttt
     6241 tgtgctcata cgttaaatct atcaccgcaa gggataaata tctaacaccg tgcgtgttga
     6301 ctattttacc tctggcggtg ataatggttg catgtactaa ggaggttgta tggaacaacg
     6361 agatgtgtat aagagacagc tggcctgccc ctcccttttg gtgtccaacc ggctcgacgg
     6421 gggcagcgca aggcggtgcc tccggcgggc cactcaatgc ttgagtatac tcactagact
     6481 ttgcttcgca aagtcgtgac cgcctacggc ggctgcggcg ccctacgggc ttgctctccg
     6541 ggcttcgccc tgcgcggtcg ctgcgctccc ttgccagccc gtggatatgt ggacgatggc
     6601 cgcgagcggc caccggctgg ctcgcttcgc tcggcccgtg gacaaccctg ctggacaagc
     6661 tgatggacag gctgcgcctg cccacgagct tgaccacagg gattgcccac cggctaccca
     6721 gccttcgacc acatacccac cggctccaac tgcgcggcct gcggccttgc cccatcaatt
     6781 tttttaattt tctctgggga aaagcctccg gcctgcggcc tgcgcgcttc gcttgccggt
     6841 tggacaccaa gtggaaggcg ggtcaaggct cgcgcagcga ccgcgcagcg gcttggcctt
     6901 gacgcgcctg gaacgaccca agcctatgcg agtgggggca gtcgaagggc gaagcccgcc
     6961 cgcctgcccc ccgagcctca cggcggcgag tgcgggggtt ccaagggggc agcgccacct
     7021 tgggcaaggc cgaaggccgc gcagtcgatc aacaagcccc ggaggggcca ctttttgccg
     7081 gagggggagc cgcgccgaag gcgtggggga accccgcagg ggtgcccttc tttgggcacc
     7141 aaagaactag atatagggcg aaatgcgaaa gacttaaaaa tcaacaactt aaaaaagggg
     7201 ggtacgcaac agctcattgc ggcacccccc gcaatagctc attgcgtagg ttaaagaaaa
     7261 tctgtaattg actgccactt ttacgcaacg cataattgtt gtcgcgctgc cgaaaagttg
     7321 cagctgattg cgcatggtgc cgcaaccgtg cggcacccct accgcatgga gataagcatg
     7381 gccacgcagt ccagagaaat cggcattcaa gccaagaaca agcccggtca ctgggtgcaa
     7441 acggaacgca aagcgcatga ggcgtgggcc gggcttattg cgaggaaacc cacggcggca
     7501 atgctgctgc atcacctcgt ggcgcagatg ggccaccaga acgccgtggt ggtcagccag
     7561 aagacacttt ccaagctcat cggacgttct ttgcggacgg tccaatacgc agtcaaggac
     7621 ttggtggccg agcgctggat ctccgtcgtg aagctcaacg gccccggcac cgtgtcggcc
     7681 tacgtggtca atgaccgcgt ggcgtggggc cagccccgcg accagttgcg cctgtcggtg
     7741 ttcagtgccg ccgtggtggt tgatcacgac gaccaggacg aatcgctgtt ggggcatggc
     7801 gacctgcgcc gcatcccgac cctgtatccg ggcgagcagc aactaccgac cggccccggc
     7861 gaggagccgc ccagccagcc cggcattccg ggcatggaac cagacctgcc agccttgacc
     7921 gaaacggagg aatgggaacg gcgcgggcag cagcgcctgc cgatgcccga tgagccgtgt
     7981 tttctggacg atggcgagcc gttggagccg ccgacacggg tcacgctgcc gcgccggtag
     8041 cacttgggtt gcgcagcaac ccgtaagtgc gctgttccag actatcggct gtaccgcctc
     8101 g
//
