LOCUS       JX560328                3123 bp    DNA     circular SYN 02-OCT-2014
DEFINITION  Cloning vector pSEVA231, complete sequence.
ACCESSION   JX560328
VERSION     JX560328.1  GI:410073675
KEYWORDS    .
SOURCE      Cloning vector pSEVA231
  ORGANISM  Cloning vector pSEVA231
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 3123)
  AUTHORS   Silva-Rocha,R., Martinez-Garcia,E., Chavarria,M., Calles,B.,
            Arce-Rodriguez,A., de las Heras,A., Paez-Espino,D.,
            Durante-Rodriguez,G., Kim,J., Nikel,P.I., Platero,R. and de
            Lorenzo,V.
  TITLE     The Standard European Vector Architecture (SEVA): a coherent
            platform for analysis and deployment of complex prokaryotic
            phenotypes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3123)
  AUTHORS   Silva-Rocha,R., Martinez-Garcia,E., Chavarria,M., Calles,B.,
            Arce-Rodriguez,A., de las Heras,A., Paez-Espino,D.,
            Durante-Rodriguez,G., Kim,J., Nikel,P.I., Platero,R. and de
            Lorenzo,V.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-AUG-2012) Systems Biology Program, Centro Nacional de
            Biotecnologia-CSIC, C/ Darwin 3, Campus de Cantoblanco, Madrid,
            Madrid 28049, Spain
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: ApE v. v2.0.40
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7902
                     /dnas_title="pSEVA231-MAGE"
                     /organism="Cloning vector pSEVA231"
                     /mol_type="other DNA"
                     /db_xref="taxon:1246706"
     primer_bind     complement(4900..4923)
                     /note="F24"
     regulatory      4930..5032
                     /regulatory_class="terminator"
                     /note="T0"
     gene            5160..5975
                     /dnas_title="neo"
                     /gene="neo"
     CDS             5160..5975
                     /dnas_title="neo"
                     /gene="neo"
                     /codon_start=1
                     /transl_table=11
                     /product="neomycin phosphotransferase"
                     /protein_id="AFV59774.1"
                     /db_xref="GI:410073676"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLY
                     GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIP
                     GKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGL
                     VDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVG
                     RVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     misc_feature    6007..6252
                     /note="oriT"
     gene            complement(6261..6923)
                     /dnas_title="rep"
                     /gene="rep"
     CDS             complement(6261..6923)
                     /dnas_title="rep"
                     /gene="rep"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="AFV59775.1"
                     /db_xref="GI:410073677"
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLL
                     HHLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAY
                     VVNDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGP
                     GEEPPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLP
                     RR"
     regulatory      7797..7901
                     /regulatory_class="terminator"
                     /note="T1"
     CDS             complement(901..2748)
                     /note="mutL E32K"
                     /translation=""
     RBS             2749..2765
                     /note="RBS"
     variation       complement(2653..2655)
                     /note="E32K mutation"
     gene            complement(2769..3446)
                     /codon_start=exo
                     /note="Exo"
                     /note="color: #a6acb3"
                     /translation="MTPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEVHNVIAKP
                     RSGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARTLFEFTSGVNVTE
                     SPIIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQY
                     SMWVTRKNAWYFANYDPRMKREGLHYVVIERDEKYMASFDEIVPEFIEKMDEALAEIG
                     FVFGEQWR"
     gene            complement(3446..4228)
                     /codon_start=bet
                     /note="Bet"
                     /note="color: #a6acb3"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQF
                     IALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNE
                     SCTCRIYRKDRNHPICVTEWMDECRREPFKTREGREITGPWQSHPKRMLRHKAMIQCA
                     RLAFGFAGIYDKDEAERIVENTAYTAERQPERDITPVNDETMQEINTLLIALDKTWDD
                     DLLPLCSQIFRRDIRASSELTQAEAVKALGFLKQKAAEQKVAA"
     gene            complement(4237..4530)
                     /codon_start=gam
                     /note="open reading frame#1"
                     /note="color: #a6acb3"
                     /translation="MNAYYIQDRLEAQSWARHYQQLAREEKEAELADDMEKGLPQHLF
                     ESLCIDHLQRHGASKKSITRAFDDDVEFQERMAEHIRYMVETIAHHQVDIDSEV"
     promoter        4600..4644
                     /note="pL promoter"
     primer_bind     4857..4879
                     /note="ARCK1"
     gene            complement(9..719)
                     /codon_start=cI repressor
                     /note="open reading frame#1"
                     /note="color: #a6acb3"
                     /translation="MSTKKKPLTQEQLEDARRLKAIYEKKKNELGLSQESVADKMGMG
                     QSGVGALFNGINALNAYNAALLTKILKVSVEEFSPSIAREIYEMYEAVSMQPSLRSEY
                     EYPVFSHVQAGMFSPKLRTFTKGDAERWVSTTKKASDSAFWLEVEGNSMTAPTGSKPS
                     FPDGMLILVDPEQAVEPGDFCIARLGGDEFTFKKLIRDSGQVFLQPLNPQYPMIPCNE
                     SCSVVGKVIASQWPEETFG"
     primer_bind     75..94
                     /note="ARCK2"
     misc_feature    790..871
                     /note="primer for cro-pBBR1"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    831..852
                     /note="final red retrieve"
                     /note="color: #a6acb3"
     rep_origin      853..872
                     /note="pBBRI ORIGIN"
                     /note="color: #a6acb3"
     PCR_primer      2659..2682
                     /note="mutL31S"
     PCR_primer      2639..2658
                     /note="mutL32F"
ORIGIN      
        1 ttaattaagc caaacgtctc ttcaggccac tgactagcga taactttccc cacaacggaa
       61 caactctcat tgcatgggat cattgggtac tgtgggttta gtggttgtaa aaacacctga
      121 ccgctatccc tgatcagttt cttgaaggta aactcatcac ccccaagtct ggctatgcag
      181 aaatcacctg gctcaacagc ctgctcaggg tcaacgagaa ttaacattcc gtcaggaaag
      241 cttggcttgg agcctgttgg tgcggtcatg gaattacctt caacctcaag ccagaatgca
      301 gaatcactgg cttttttggt tgtgcttacc catctctccg catcaccttt ggtaaaggtt
      361 ctaagcttag gtgagaacat ccctgcctga acatgagaaa aaacagggta ctcatactca
      421 cttctaagtg acggctgcat actaaccgct tcatacatct cgtagatttc tctggcgatt
      481 gaagggctaa attcttcaac gctaactttg agaatttttg taagcaatgc ggcgttataa
      541 gcatttaatg cattgatgcc attaaataaa gcaccaacgc ctgactgccc catccccatc
      601 ttgtctgcga cagattcctg ggataagcca agttcatttt tctttttttc ataaattgct
      661 ttaaggcgac gtgcgtcctc aagctgctct tgtgttaatg gtttcttttt tgtgctcata
      721 cgttaaatct atcaccgcaa gggataaata tctaacaccg tgcgtgttga ctattttacc
      781 tctggcggtg ataatggttg catgtactaa ggaggttgta tggaacaacg agatgtgtat
      841 aagagacagc tggcctgccc ctcccttttg gttgggatcc tctagagtcT CATCGCCATT
      901 TCACTCATCT TTCAGGGCTT TTATCGCCGG ATGTAAATCA ACAGATTGTA ACAGACCACC
      961 CGGCGGCGTT TTCACAAGTT GCGGACATAA CCGTTCCACG TCCGCCAGCA GGGTTATGGC
     1021 CTGTGCCATT GACCACTGCG CATGTTCGCT CATCAGATTT CGTGCAATCC ACTGCGCAAT
     1081 ATTGCCAGGT TCGAATACGG ACTGCTTCGC CAGGTAGCCT ATCAGTTCAG GAATCAAGAT
     1141 TTGTAAATTT TGTTGGCGTA AGGGTAAAGG CACTGCCCTG ATGGTCACAT GCTGTGCATC
     1201 TGACTGGAAA TCAATACCCA ATTCCGCCAG GGCAGACTGC GCTTTTTCTA ATGCCGATTT
     1261 TTCTTCGGCA GAAACTTTTA GCCGCAACGG AATCAGCAGC GGCTGGGCGC AAACGGGCGC
     1321 TTCACCCGGC GTCAATTGTG CCTGACGCAG CCAACGTTCT GCCACTGGCA AGGATAAAAG
     1381 TGAAATGTTG CCGTCGCGCT CCAGCAACGC ACAGTCGGAA TGGACGATAG TCAGTACCCG
     1441 ACCAAAACTC TGACTGTTCG CCGCAAGTGC AGGTTCCTGC GGTTCCGGCG CTTTTAATTT
     1501 TTGCATCGGC GCGGGCGTTT GCAAAAGCTG GCGATACACT TCACCTTGCT GTTTCTGGTA
     1561 GCCTGGCTGC GCATTCGGCC AGGGGGCAGC CGGACGACTG CCTGATGCTG GCGCAGGAGT
     1621 GTAGCGCGGA GCTACCGGCT CACGAGCTGC CGGTTCTGCA AAGTGATTGC GCCCCGCCGC
     1681 CACGCGGTTT TCCGGAATGG AACGCGGTGC AGGTTGGGGT TCATCGTCCA GCGGTAGCGG
     1741 CGTTTCCAGT TGCTGTTGTA GCACGCTCAG CACGCCCTGA TAGATAAAAT CATGCACCAG
     1801 ACGCGACTGA TGGAAACGCA CTTCGTGTTT GGCGGGGTGC ACGTTGACGT CCACCTGATG
     1861 TGGGTCGATC TCCAGATACA ACACAAATGC CGGTTGCTGA TCGGCCCCCA GTTTGTCTTC
     1921 GCAGGCCTGG CGGATCGCGT GATTGATCAG GCGATCGCGC ATCATGCGAC CGTTCACGTA
     1981 GCAATACTGA ATTTCTGCCA GTGCGGGCGT GGTGTGATTT GGATCGGCCA CCCAGCCGCG
     2041 TAGCGTGAGA TCGCCGTGTT GCCATTCAAT CGCCAGCGCT TGTTCAAGAA AAGCGGTGCC
     2101 GCAAATCGCG CCTAAGCGCC GTTCTTTTTG CCCGCCTTCC GGCACTGCGC GGTACTGACG
     2161 CACAATTTTA CCGTTATGCG ACAGGTTGAT CGTGACGTCG AAACGCGCCA GCGCAATGCG
     2221 GCGGATGATC TCATCAATGT GGTTAAATTC GGTTTTCTCG GTGCGCAGGA ATTTGCGCCG
     2281 CGCCGGGGTG TTGTAGAACA GATCCAGCAC CTCCAGCGTC GTCCCCACAG GATGCGCCGC
     2341 CGGTTTTACC GTCACGTTCA TATCGCGCCC TTCGGCATAG GCCTGCCAGG CTTCCTGCTG
     2401 TTCTGCGGTG CGTGAAGTGA GCGTCAGGCG GGAAACCGAA CTGATACTCG CCAGCGCCTC
     2461 ACCGCGAAAG CCCAGGCTGA TAATGGCTTC GAGATCGTCC AGAGAGGCGA TTTTACTGGT
     2521 GGCATGACGA GCCAGCGCCA GCGCCAGCTC ATCTTTTTTG ATACCGCAGC CGTTATCACG
     2581 AATGCGGATA AGTTTCGCCC CACCGCGTTC GATATCAATA TCGATACGCG TCGCACCTGC
     2641 ATCGAGGCTG TTTTtCACcA GTTCTTTGAC TACCGACGCA GGTCGCTCGA CCACCTCACC
     2701 TGCGGCAATC TGGTTCGCCA GTTGTGGCGG TAAGACCTGA ATTGGCATTT TGTAATCCTC
     2761 CTCTCtcatc gccattgctc cccaaataca aaaccaattt cagccagtgc ctcgtccatt
     2821 ttttcgatga actccggcac gatctcgtca aaactcgcca tgtacttttc atcccgctca
     2881 atcacgacat aatgcaggcc ttcacgcttc atacgcgggt catagttggc aaagtaccag
     2941 gcattttttc gcgtcaccca catgctgtac tgcacctggg ccatgtaagc tgactttatg
     3001 gcctcgaaac caccgagccg gaacttcatg aaatcccggg aggtaaacgg gcatttcagt
     3061 tcaaggccgt tgccgtcact gcataaacca tcgggagagc aggcggtacg catactttcg
     3121 tcgcgataga tgatcgggga ttcagtaaca ttcacgccgg aagtgaactc aaacagggtt
     3181 ctggcgtcgt tctcgtactg ttttccccag gccagtgctt tagcgttaac ttccggagcc
     3241 acaccggtgc aaacctcagc aagcagggtg tggaagtagg acattttcat gtcaggccac
     3301 ttctttccgg agcggggttt tgctatcacg ttgtgaactt ctgaagcggt gatgacgccg
     3361 agccgtaatt tgtgccacgc atcatccccc tgttcgacag ctctcacatc gatcccggta
     3421 cgctgcagga taatgtccgg tgtcatgctg ccaccttctg ctctgcggct ttctgtttca
     3481 ggaatccaag agcttttact gcttcggcct gtgtcagttc tgacgatgca cgaatgtcgc
     3541 ggcgaaatat ctgggaacag agcggcaata agtcgtcatc ccatgtttta tccagggcga
     3601 tcagcagagt gttaatctcc tgcatggttt catcgttaac cggagtgatg tcgcgttccg
     3661 gctgacgttc tgcagtgtat gcagtatttt cgacaatgcg ctcggcttca tccttgtcat
     3721 agataccagc aaatccgaag gccagacggg cacactgaat catggcttta tgacgtaaca
     3781 tccgtttggg atgcgactgc cacggccccg tgatttctct gccttcgcga gttttgaatg
     3841 gttcgcggcg gcattcatcc atccattcgg taacgcagat cggatgatta cggtccttgc
     3901 ggtaaatccg gcatgtacag gattcattgt cctgctcaaa gtccatgcca tcaaactgct
     3961 ggttttcatt gatgatgcgg gaccagccat caacgcccac caccggaacg atgccattct
     4021 gcttatcagg aaaggcgtaa atttctttcg tccacggatt aaggccgtac tggttggcaa
     4081 cgatcagtaa tgcgatgaac tgcgcatcgc tggcatcacc tttaaatgcc gtctggcgaa
     4141 gagtggtgat cagttcctgt gggtcgacag aatccatgcc gacacgttca gccagcttcc
     4201 cagccagcgt tgcgagtgca gtactcattc gttttatacc tctgaatcaa tatcaacctg
     4261 gtggtgagca atggtttcaa ccatgtaccg gatgtgttct gccatgcgct cctgaaactc
     4321 aacatcgtca tcaaacgcac gggtaatgga ttttttgctg gccccgtggc gttgcaaatg
     4381 atcgatgcat agcgattcaa acaggtgctg gggcaggcct ttttccatgt cgtctgccag
     4441 ttctgcctct ttctcttcac gggcgagctg ctggtagtga cgcgcccagc tctgagcctc
     4501 aagacgatcc tgaatgtaat aagcgttcat ggctgaactc ctgaaatagc tgtgaaaata
     4561 tcgcccgcga aatgccgggc tgattagtaa tccggaatcg cacttacggc caatgcttcg
     4621 tttcgtatca cacaccccaa agccttctgc tttgaatgct gcccttcttc agggcttaat
     4681 ttttaagagc gtcaccttca tggtggtcag tgcgtcctgc tgatgtgctc agtatcaccg
     4741 ccagtggtat ttatgtcaac accgccagag ataatttatc accgcagatg gttatctgta
     4801 tgttttttat atgaatttat tttttgcagg ggggcattgt ttggtaggtg agagatctga
     4861 attgctatgt ttagtgagtg catgcaagct tgcggccgcg tcgtgactgg gaaaaccctg
     4921 gcgactagtc ttggactcct gttgatagat ccagtaatga cctcagaact ccatctggat
     4981 ttgttcagaa cgctcggttg ccgccgggcg ttttttattg gtgagaatcc aggggtcccc
     5041 aataattacg atttaaattt gtgtctcaaa atctctgatg ttacattgca caagataaaa
     5101 atatatcatc atgaacaata aaactgtctg cttacataaa cagtaataca aggggtgtta
     5161 tgagccatat tcagcgtgaa acgagctgta gccgtccgcg tctgaacagc aacatggatg
     5221 cggatctgta tggctataaa tgggcgcgtg ataacgtggg tcagagcggc gcgaccattt
     5281 atcgtctgta tggcaaaccg gatgcgccgg aactgtttct gaaacatggc aaaggcagcg
     5341 tggcgaacga tgtgaccgat gaaatggtgc gtctgaactg gctgaccgaa tttatgccgc
     5401 tgccgaccat taaacatttt attcgcaccc cggatgatgc gtggctgctg accaccgcga
     5461 ttccgggcaa aaccgcgttt caggtgctgg aagaatatcc ggatagcggc gaaaacattg
     5521 tggatgcgct ggccgtgttt ctgcgtcgtc tgcatagcat tccggtgtgc aactgcccgt
     5581 ttaacagcga tcgtgtgttt cgtctggccc aggcgcagag ccgtatgaac aacggcctgg
     5641 tggatgcgag cgattttgat gatgaacgta acggctggcc ggtggaacag gtgtggaaag
     5701 aaatgcataa actgctgccg tttagcccgg atagcgtggt gacccacggc gattttagcc
     5761 tggataacct gattttcgat gaaggcaaac tgattggctg cattgatgtg ggccgtgtgg
     5821 gcattgcgga tcgttatcag gatctggcca ttctgtggaa ctgcctgggc gaatttagcc
     5881 cgagcctgca aaaacgtctg tttcagaaat atggcattga taatccggat atgaacaaac
     5941 tgcaatttca tctgatgctg gatgaatttt tctaataatt aattggaccg cggtccgcgc
     6001 gttgtccttt tccgctgcat aaccctgctt cggggtcatt atagcgattt tttcggtata
     6061 tccatccttt ttcgcacgat atacaggatt ttgccaaagg gttcgtgtag actttccttg
     6121 gtgtatccaa cggcgtcagc cgggcaggat aggtgaagta ggcccacccg cgagcgggtg
     6181 ttccttcttc actgtccctt attcgcacct ggcggtgctc aacgggaatc ctgctctgcg
     6241 aggctggccg taggccggcc ctaccggcgc ggcagcgtta cccgtgtcgg cggctccaac
     6301 ggctcgccat cgtccagaaa acacggctca tcgggcatcg gcaggcgctg ctgcccgcgc
     6361 cgttcccatt cctccgtttc ggtcaaggct ggcaggtctg gttccatgcc cggaatgccg
     6421 ggctggctgg gcggctcctc gccggggccg gtcggtagtt gctgctcgcc cggatacagg
     6481 gtcgggatgc ggcgcaggtc gccatgcccc aacagcgatt cgtcctggtc gtcgtgatca
     6541 accaccacgg cggcactgaa caccgacagg cgcaactggt cgcggggctg gccccacgcc
     6601 acgcggtcat tgaccacgta ggccgacacg gtgccggggc cgttgagctt cacgacggag
     6661 atccagcgct cggccaccaa gtccttgact gcgtattgga ccgtccgcaa agaacgtccg
     6721 atgagcttgg aaagtgtctt ctggctgacc accacggcgt tctggtggcc catctgcgcc
     6781 acgaggtgat gcagcagcat tgccgccgtg ggtttcctcg caataagccc ggcccacgcc
     6841 tcatgcgctt tgcgttccgt ttgcacccag tgaccgggct tgttcttggc ttgaatgccg
     6901 atttctctgg actgcgtggc catgcttatc tccatgcggt aggggtgccg cacggttgcg
     6961 gcaccatgcg caatcagctg caacttttcg gcagcgcgac aacaattatg cgttgcgtaa
     7021 aagtggcagt caattacaga ttttctttaa cctacgcaat gagctattgc ggggggtgcc
     7081 gcaatgagct gttgcgtacc cccctttttt aagttgttga tttttaagtc tttcgcattt
     7141 cgccctatat ctagttcttt ggtgcccaaa gaagggcacc cctgcggggt tcccccacgc
     7201 cttcggcgcg gctccccctc cggcaaaaag tggcccctcc ggggcttgtt gatcgactgc
     7261 gcggccttcg gccttgccca aggtggcgct gcccccttgg aacccccgca ctcgccgccg
     7321 tgaggctcgg ggggcaggcg ggcgggcttc gcccttcgac tgcccccact cgcataggct
     7381 tgggtcgttc caggcgcgtc aaggccaagc cgctgcgcgg tcgctgcgcg agccttgacc
     7441 cgccttccac ttggtgtcca accggcaagc gaagcgcgca ggccgcaggc cggaggcttt
     7501 tccccagaga aaattaaaaa aattgatggg gcaaggccgc aggccgcgca gttggagccg
     7561 gtgggtatgt ggtcgaaggc tgggtagccg gtgggcaatc cctgtggtca agctcgtggg
     7621 caggcgcagc ctgtccatca gcttgtccag cagggttgtc cacgggccga gcgaagcgag
     7681 ccagccggtg gccgctcgcg gccatcgtcc acatatccac gggctggcaa gggagcgcag
     7741 cgaccgcgca gggcgaagcc cggagagcaa gcccgtaggg ggggcgcgcc cagctgtcta
     7801 gggcggcgga tttgtcctac tcaggagagc gttcaccgac aaacaacaga taaaacgaaa
     7861 ggcccagtct ttcgactgag cctttcgttt tatttgatgc ct
//

